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KMID : 0984920180200020051
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Journal of Skin Barrier Research
2018 Volume.20 No. 2 p.51 ~ p.51
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Microfluidic Skin Chip Co-cultured with Vascular Structure and Immune Cells for Recapitulating Dermal Immune Response
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Kim Seung-Ri
Sung Jong-Hwan
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Abstract
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Current in vitro skin models do not recapitulate the complex architecture and functions of the skin tissue. In particular, construction of an in vitro model comprising the epidermis and dermis layer with vascular structure for mass transport and immune response has not been reported yet. In this study, we aim to develop a microfluidic, three-dimensional (3D) skin chip with fluidic channels using PDMS and hydrogels. Mass transport-reaction model of oxygen and glucose inside the skin chip was developed to aid the design of the microfluidic skin chip. Evaluation of viabilities of dermal fibroblasts and HaCaT cultured in the chip verified that the presence of flow plays a crucial role in maintaining the viability, and we were able to observe migration of immune cells and secretion of cytokines in response to external stimulation by UV or chemicals. Our 3D skin chip with vascular structures can be a valuable in vitro model for reproducing the interaction between different components of the skin tissue such as immune response and transport of nutrients, and may work as a more physiologically realistic platform for testing skin reaction to cosmetic products and drugs.
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